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nf-core/popscle/dscpileup @ 0.0.0-0c7146d

Software to pileup reads and corresponding base quality for each overlapping SNPs and each barcode.

Latest version: 0.0.0-0c7146d
Total downloads: 1
Source: nf-core/modules

Summary

Software to pileup reads and corresponding base quality for each overlapping SNPs and each barcode.

Get started

Add the following snippet to your workflow script to include this module.

include { POPSCLE_DSCPILEUP } from 'nf-core/popscle/dscpileup'

License

MIT License

Process
Name POPSCLE_DSCPILEUP
Input 1 channel
#1 tuple
meta map

Groovy Map containing sample information e.g. [ id:'sample1' ]

bam file

Input SAM/BAM/CRAM file produced by the standard 10x sequencing platform, or any other barcoded single cell RNA-seq.

*.{bam,cram,sam}
vcf file

Input VCF/BCF file files containing (AC) and (AN) from referenced population (e.g. 1000g).

*.{vcf,bcf}
Output 5 channels
#1 cel tuple
meta map

Groovy Map containing sample information e.g. [ id:'sample1']

*.cel.gz file

Contains the relation between numerated barcode ID and barcode and the number of SNP and number of UMI for each barcoded droplet.

*.cel.gz
#2 plp tuple
meta map

Groovy Map containing sample information e.g. [ id:'sample1']

*.plp.gz file

Contains the overlapping SNP and the corresponding read and base quality for each barcode ID.

*.plp.gz
#3 umi tuple
meta map

Groovy Map containing sample information e.g. [ id:'sample1']

*.umi.gz file

Contains the position covered by each umi.

*.umi.gz
#4 var tuple
meta map

Groovy Map containing sample information e.g. [ id:'sample1']

*.var.gz file

Contains the position, reference allele and allele frequency for each SNP.

*.var.gz
#5 versions
versions.yml file

File containing software versions

versions.yml
Tool Description Homepage
popscle A suite of population scale analysis tools for single-cell genomics data including implementation of Demuxlet / Freemuxlet methods and auxiliary tools https://github.com/statgen/popscle
Version 0.0.0-0c7146d
Commit ID 135d883a43b1a2324b4112cd270cf22e20835533
Release Date 08 Apr 2026 19:04:53 (UTC)
Download URL https://registry-stage.nextflow.io/api/v1/modules/nf-core%2Fpopscle%2Fdscpileup/0.0.0-0c7146d/download
OCI Store URL https://public.cr.stage-seqera.io/v2/nextflow/plugin-stage/modules/nf-core/popscle/dscpileup/blobs/sha256:f3e6c87fa1ea165562d7a914bece37e08245a8a6cfad117a0bbd9d6493319a04
Size 2.7 KB
Checksum sha256:f3e6c87fa1ea165562d7a914bece37e08245a8a6cfad117a0bbd9d6493319a04
Downloads 1
Version Date Status Downloads Size Diff
0.0.0-0c7146d 08 Apr 2026 19:04:53 (UTC) 1 2.7 KB -