Nextflow Registry | nf-core/popscle/demuxlet@0.0.0-0c7146d

nf-core/popscle/demuxlet @ 0.0.0-0c7146d

Software to deconvolute sample identity and identify multiplets when multiple samples are pooled by barcoded single cell sequencing and external genotyping data for each sample is available.

popscle demultiplexing genotype-based deconvoltion single cell

Latest version: 0.0.0-0c7146d

Total downloads: 1

Source: nf-core/modules

Authors: @mari-ga @maxozo @wxicu @Zethson

Maintainers: @mari-ga @maxozo @wxicu @Zethson

Summary

Software to deconvolute sample identity and identify multiplets when multiple samples are pooled by barcoded single cell sequencing and external genotyping data for each sample is available.

Get started

Add the following snippet to your workflow script to include this module.

include { POPSCLE_DEMUXLET } from 'nf-core/popscle/demuxlet'

License

MIT License

Process

`Name`	`POPSCLE_DEMUXLET`

Input 1 channel

#1 tuple

`meta` map	Groovy Map containing sample information e.g. `[ id:'sample1']`
`plp_prefix` string	Prefix of pileup files (CEL,VAR and PLP) produced by popscle/dsc_pileup.
`bam` file	Input SAM/BAM/CRAM file without running popscle/dsc_pileup, must be sorted by coordinates and indexed. `*.{bam,cram,sam}`
`donor_genotype` file	Input VCF/BCF file, containing the individual genotypes (GT), posterior probability (GP), or genotype likelihood (PL) to assign each barcode to a specific sample (or a pair of samples) in the VCF file. `*.{vcf,bcf}`

Output 2 channels

#1 versions

`versions.yml` file	File containing software versions `versions.yml`

#2 demuxlet_result tuple

`meta` map	Groovy Map containing sample information e.g. `[ id:'sample1']`
`*.best` file	Result of demuxlet containing the best guess of the sample identity, with detailed statistics to reach to the best guess. `*.best`

Tool	Description	Homepage
popscle	A suite of population scale analysis tools for single-cell genomics data including implementation of Demuxlet / Freemuxlet methods and auxiliary tools	https://github.com/statgen/popscle

Version	0.0.0-0c7146d
Commit ID	135d883a43b1a2324b4112cd270cf22e20835533
Release Date	08 Apr 2026 19:04:51 (UTC)
Download URL	https://registry-stage.nextflow.io/api/v1/modules/nf-core%2Fpopscle%2Fdemuxlet/0.0.0-0c7146d/download
OCI Store URL	https://public.cr.stage-seqera.io/v2/nextflow/plugin-stage/modules/nf-core/popscle/demuxlet/blobs/sha256:a7a219d7077b06d8adc0b1faa6f46b73e1d4bec01fe9f1ce9fb51ddc45f62766
Size	2.9 KB
Checksum	sha256:a7a219d7077b06d8adc0b1faa6f46b73e1d4bec01fe9f1ce9fb51ddc45f62766
Downloads	1

Version	Date	Status	Downloads	Size	Diff
0.0.0-0c7146d	08 Apr 2026 19:04:51 (UTC)		1	2.9 KB	-