nf-core/backsub @ 0.0.0-0c7146d
Summary
Pixel-by-pixel channel subtraction tool for multiplexed immunofluorescence data.
Get started
Add the following snippet to your workflow script to include this module.
include { BACKSUB } from 'nf-core/backsub'
License
MIT License
Process
Name
|
BACKSUB |
|---|
Input
2 channels
#1
tuple
meta
map
|
Groovy Map containing sample information e.g. [ id:'test' ] |
|---|---|
image
file
|
Multi-channel image file *.{tif,tiff}
|
#2
tuple
meta2
map
|
Groovy Map containing sample information e.g. [ id:'test' ] |
|---|---|
markerfile
file
|
Marker file with channel names, exposure times, and specified background to subtract (and remove to exclude channels from output) *.csv
|
Output
3 channels
#1
markerout
tuple
meta2
map
|
Groovy Map containing sample information e.g. [ id:'test' ] |
|---|---|
*.csv
file
|
Marker file adjusted to match the background corrected image *.{csv}
|
#2
backsub_tif
tuple
meta
map
|
Groovy Map containing sample information e.g. [ id:'test' ] |
|---|---|
*.ome.tif
file
|
Background corrected pyramidal ome.tif *.{tif}
|
#3
versions_backsub
tuple
${task.process}
string
|
The name of the process |
|---|---|
backsub
string
|
The name of the tool |
backsub --version
eval
|
The expression to obtain the version of the tool |
| Tool | Description | Homepage |
|---|---|---|
| backsub | Pixel-by-pixel channel subtraction tool for multiplexed immunofluorescence data | https://github.com/SchapiroLabor/Background_subtraction |
| Version | 0.0.0-0c7146d |
|---|---|
| Commit ID | 0c7146d85582628b5d1034504702fa808632b1af |
| Release Date | 08 Apr 2026 18:23:27 (UTC) |
| Download URL | https://registry-stage.nextflow.io/api/v1/modules/nf-core%2Fbacksub/0.0.0-0c7146d/download |
| OCI Store URL | https://public.cr.stage-seqera.io/v2/nextflow/plugin-stage/modules/nf-core/backsub/blobs/sha256:f66bbacdca0d80ec80f10de40aed0462ea2173231a61791f19a26b7dfe6a6d16 |
| Size | 2.7 KB |
| Checksum | sha256:f66bbacdca0d80ec80f10de40aed0462ea2173231a61791f19a26b7dfe6a6d16 |
| Downloads | 1 |
| Version | Date | Status | Downloads | Size | Diff |
|---|---|---|---|---|---|
| 0.0.0-0c7146d | 08 Apr 2026 18:23:27 (UTC) | 1 | 2.7 KB | - |